J. David Legan, PhD
Director of Science
David earned his Ph.D. in Food Technology from the University of Reading in the UK by modeling the ecology of mixed microbial populations, and then moved to Campden BRI in a variety of microbiological food safety research and client service roles. During that time, he was project lead for the Bacillus component of the UK’s pathogen modeling program. He moved again to Nabisco Research in New Jersey where he ran the corporate microbiology lab and developed a program of preservation technology development and microbial modeling. After the Kraft Foods acquisition, he moved to Chicago to work on Food Safety and Preservation research, and through modeling and validation studies:
- Optimized Oscar Mayer’s use of lactate and diacetate and their naturally cultured alternatives as Listeria-control agents in Ready to Eat meats
- Specified process conditions central to Oscar Mayer’s commercial launch of High Pressure Pasteurization of naturally cured RTE meats
David had responsibility for the Kraft cultures R&D group, developed a partnership to explore microwave sterilization leading to several patents, and led a program that developed an internal proprietary natural antimicrobial commercialized in several Kraft products. Technologies from his group supported approximately $4 billion in annual sales.
After years as a microbiology "client", he is now back in the "provider" role as Director of Science at Eurofins Microbiology Laboratories, Inc., by way of the Covance Food Solutions group based in Madison, WI, which he joined in 2016. In this role, he ensures appropriate method validation, explores new testing technologies, and fields multiple complicated food microbiology questions.
Products that his team has evaluated or developed and launched include:
- The 3M MDS platform in the Madison microbiology laboratory
- Flow cytometry for enumeration of probiotics
- Strain-level confirmation of probiotic identification using the polymerase chain reaction (PCR)
- Next-generation sequencing using the Oxford Nanopore Technologies GridION sequencing platform for microbial identification and microbiome analysis
Below are resources from David:
If you are a manufacturer or sell probiotic supplements, testing for counts of probiotic strains is not always straight forward. Here are five tips you should know when it comes to testing your product and verifying your label claims.
Testing or enumeration Bacillus coagulans in gummies is not as straight forward as it seems. Sometimes product matrices are challenging and require a new custom method. Learn how our testing experts know exactly how to problem solve testing in tough matrices like gummy candies.
If you have been using an established probtioic enumeration method with good results, introducing new probiotic strains into your formulas may mean changes need to be made. Read this case study to learn how Eurofins finds a solution to challenging product with multiple strains from multiple manufacturers.
Is your probiotic supplement formulated with both probiotic bacteria and active compounds? If so, getting accurate enumeration of probiotic strains can be a challenge. Learn how our experts in probiotic testing are skilled in finding solutions to tough probiotic formulations in this case study.
New Rapid Pathogen Testing Services. Eurofins Microbiology Laboratories, Inc. is pleased to share our new Enteric Pathogen Risk Indicator (EPRI™) Testing. This new service is a rapid approach to risk identification and management using fast genetic technology (PCR) with the benefits of an indicator test. In addition, we can screen for Salmonella and Shiga toxigenic E. coli at the same time in one assay.
Validation of aseptic fillers intended for the production of low-acid, shelf-stable products is a core requirement of the FDA’s low-acid canned food (LACF) regulations (21CFR113 and 108.35). Microbiological challenges of the aseptic zone and packaging material sterilization cycles are important components of the validation process. These challenges are often conducted at the production site using non-pathogenic surrogate organisms that mimic the resistance of the pertinent target pathogen. This presentation will provide a brief history of the use of such surrogates for the validation of low-acid aseptic fillers along with the approach we have used to qualify the surrogates as adequate for the intended technology.”
When we send a sample of food for testing, we generally think about a pretty simple system where we submit a sample, wait a bit of time, and then find out if the target organism is there. What we often forget is just how complex our food, processing systems, and environments truly are.
Join our team of presenters at Eurofins Microbiology Laboratories for an in-depth webinar all about rapid microbiological methods. They presented on the range of methods available, as well as the technologies behind them, while also exploring factors that affect their performance. Learn what to consider when selecting a rapid method such as sensitivity, validation status, and speed. Practical considerations around the method applications will be addressed.
Learn about the most common food pathogens and their impact in food handling and production. Testing your products can help manage risk of contamination from pathogens. Partner with Eurofins for all of your food safety testing needs.
New validated methods are here at Eurofins! Check out this white paper written by Joelle Mosso on our new method for faster and more cost efficient pathogen analysis on beef trim using MicroTally® cloths!
