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J. David Legan

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J. David Legan, PhD

J. David Legan, PhD

Director of Science

David earned his Ph.D. in Food Technology from the University of Reading in the UK by modeling the ecology of mixed microbial populations, and then moved to Campden BRI in a variety of microbiological food safety research and client service roles. During that time, he was project lead for the Bacillus component of the UK’s pathogen modeling program.  He moved again to Nabisco Research in New Jersey where he ran the corporate microbiology lab and developed a program of preservation technology development and microbial modeling.  After the Kraft Foods acquisition, he moved to Chicago to work on Food Safety and Preservation research, and through modeling and validation studies:

  • Optimized Oscar Mayer’s use of lactate and diacetate and their naturally cultured alternatives as Listeria-control agents in Ready to Eat meats
  • Specified process conditions central to Oscar Mayer’s commercial launch of High Pressure Pasteurization of naturally cured RTE meats

David had responsibility for the Kraft cultures R&D group, developed a partnership to explore microwave sterilization leading to several patents, and led a program that developed an internal proprietary natural antimicrobial commercialized in several Kraft products. Technologies from his group supported approximately $4 billion in annual sales.

After years as a microbiology "client", he is now back in the "provider" role as Director of Science at Eurofins Microbiology Laboratories, Inc., by way of the Covance Food Solutions group based in Madison, WI, which he joined in 2016.  In this role, he ensures appropriate method validation, explores new testing technologies, and fields multiple complicated food microbiology questions.

Products that his team has evaluated or developed and launched include:

  • The 3M MDS platform in the Madison microbiology laboratory
  • Flow cytometry for enumeration of probiotics
  • Strain-level confirmation of probiotic identification using the polymerase chain reaction (PCR)
  • Next-generation sequencing using the Oxford Nanopore Technologies GridION sequencing platform for microbial identification and microbiome analysis

 

Below are resources from David:



A large dairy product manufacturer expressed interest in an alternate PCR method for the detection of Genus Listeria in their environmental and product testing program due to recurring false-positive detections and high levels of environmental Genus Listeria positives in the recent past. Our objective was to compare the performance of two PCR based methods for Genus Listeria detection.


This scientific poster demonstrates how flow cytometry can be used to accurately and precisely enumerate B. coagulans and B. subtilis in a gummy matrix.


Third-party food safety testing is a surefire strategy to save money, boost quality, and increase profit margins. Find out how Eurofins can partner with your company.


Our objective was to compare the efficacy of commercially available pathogen screening platforms for detecting Salmonella in probiotic cultures to permit evidence-based platform selection.


This poster explores the use of flow cytometry in GFP-tagged Salmonella strains as positive controls for rapid qualitative Salmonella detection testing.


This poster demonstrates how DNA sequencing can be an important tool in quality control, presumptive confirmations, and environmental monitoring.


This infographic poster describes how targeted and shotgun metagenome-based microbiomes use DNA sequencing to identify and provide proportions of microorganisms in a single assay.


Researchers from Eurofins Microbiology and Third Wave Bioactives collaborated to compare the microbial succession of refrigerated chicken noodle soup. The purpose of better understanding how various culture ingredients not only prevent microbial spoilage populations, but also dive deeper into how microbial populations evolve over time.


The experts at Eurofins have identified some key considerations for environment sample collection that will help ensure quality results. These quick tips will help you build a solid foundation for detecting facility risks as you conduct your environmental monitoring programs.


This study seeks to establish the detectability of Listeria species in probiotic cultures and estimate the sensitivity of detection.


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