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J. David Legan

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J. David Legan, PhD

J. David Legan, PhD

Director of Science

David earned his Ph.D. in Food Technology from the University of Reading in the UK by modeling the ecology of mixed microbial populations, and then moved to Campden BRI in a variety of microbiological food safety research and client service roles. During that time, he was project lead for the Bacillus component of the UK’s pathogen modeling program.  He moved again to Nabisco Research in New Jersey where he ran the corporate microbiology lab and developed a program of preservation technology development and microbial modeling.  After the Kraft Foods acquisition, he moved to Chicago to work on Food Safety and Preservation research, and through modeling and validation studies:

  • Optimized Oscar Mayer’s use of lactate and diacetate and their naturally cultured alternatives as Listeria-control agents in Ready to Eat meats
  • Specified process conditions central to Oscar Mayer’s commercial launch of High Pressure Pasteurization of naturally cured RTE meats

David had responsibility for the Kraft cultures R&D group, developed a partnership to explore microwave sterilization leading to several patents, and led a program that developed an internal proprietary natural antimicrobial commercialized in several Kraft products. Technologies from his group supported approximately $4 billion in annual sales.

After years as a microbiology "client", he is now back in the "provider" role as Director of Science at Eurofins Microbiology Laboratories, Inc., by way of the Covance Food Solutions group based in Madison, WI, which he joined in 2016.  In this role, he ensures appropriate method validation, explores new testing technologies, and fields multiple complicated food microbiology questions.

Products that his team has evaluated or developed and launched include:

  • The 3M MDS platform in the Madison microbiology laboratory
  • Flow cytometry for enumeration of probiotics
  • Strain-level confirmation of probiotic identification using the polymerase chain reaction (PCR)
  • Next-generation sequencing using the Oxford Nanopore Technologies GridION sequencing platform for microbial identification and microbiome analysis

 

Below are resources from David:



DNA sequencing is an important tool to identify all Probiotic organisms in a sample all in one assay. In this Scientific Poster, Eurofins scientists investigate a long-read sequencing method that accurately identifies probiotic organisms in complex samples recently presented at SHIFT 20.


In this poster, Eurofins describes how the utility of flow cytometry was expanded further by applying modifications to ISO 19344(B) in order to accurately quantify the yeast in the sample.


This analysis compares BACGene Real-Time PCR and BAX® System PCR Methods for the Detection of Salmonella in Pet Food.


In this case study learn more about how the unique Eurofins ExpressMicro service molded to meet the needs and became the preferred solution, saving both time and money for a leading food producer.


At Eurofins, ExpressMicro samples arrive at the lab in the early morning hours, six to eleven hours earlier than other labs—and we deliver results up to eight hours faster than standard next-day air shipping.


Eurofins Microbiology network has a one-stop-shop approach that allows you to work with one lab, receive one report, and one invoice saving you both time and money.
Keywords: Video Microbiology


With so many food pathogen detection methods available deciding which is most suitable to your process, product and facility can be difficult. Read more as Eurofins dissects the pros and cons of immunoassay and molecular methodology.


This blog discusses the importance of testing aspergillus in hemp and cbd products.


It’s no secret that hemp is a fast-growing market with applications in a wide range of products from supplements to clothing. Watch this comprehensive webinar in which Eurofins experts discuss hemp testing considerations for hemp farmers and processors including genetic, microbiological, and analytical testing.


This poster—originally presented at IFT’s annual 2019 meeting—seeks to determine the robustness of LAMP pathogen assays by testing wide variations in temperature, incubation time, and other conditions, to facilitate efficient laboratory operation in “real world” conditions.


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