J. David Legan

J. David Legan, PhD

Director of Science

David earned his Ph.D. in Food Technology from the University of Reading in the UK by modeling the ecology of mixed microbial populations, and then moved to Campden BRI in a variety of microbiological food safety research and client service roles. During that time, he was project lead for the Bacillus component of the UK’s pathogen modeling program. He moved again to Nabisco Research in New Jersey where he ran the corporate microbiology lab and developed a program of preservation technology development and microbial modeling. After the Kraft Foods acquisition, he moved to Chicago to work on Food Safety and Preservation research, and through modeling and validation studies:

Optimized Oscar Mayer’s use of lactate and diacetate and their naturally cultured alternatives as Listeria-control agents in Ready to Eat meats
Specified process conditions central to Oscar Mayer’s commercial launch of High Pressure Pasteurization of naturally cured RTE meats

David had responsibility for the Kraft cultures R&D group, developed a partnership to explore microwave sterilization leading to several patents, and led a program that developed an internal proprietary natural antimicrobial commercialized in several Kraft products. Technologies from his group supported approximately $4 billion in annual sales.

After years as a microbiology "client", he is now back in the "provider" role as Director of Science at Eurofins Microbiology Laboratories, Inc., by way of the Covance Food Solutions group based in Madison, WI, which he joined in 2016. In this role, he ensures appropriate method validation, explores new testing technologies, and fields multiple complicated food microbiology questions.

Products that his team has evaluated or developed and launched include:

The 3M MDS platform in the Madison microbiology laboratory
Flow cytometry for enumeration of probiotics
Strain-level confirmation of probiotic identification using the polymerase chain reaction (PCR)
Next-generation sequencing using the Oxford Nanopore Technologies GridION sequencing platform for microbial identification and microbiome analysis

Below are resources from David:

From Tank to Gut: Why You Need Microbiome Monitoring For Your Dairy Products

What species of microorganisms are present in your dairy products? Learn why you need microbiome monitoring for your dairy products.

Keywords: Blog Dairy Microbiome Microbiology

USP Methods for Microbial Contamination in Supplements, CBD/Hemp and Probiotics

In this presentation, USP and Eurofins Microbiology provided an overview of USP microbiology methods tests for microbial contamination in dietary supplements, CBD/hemp products, and probiotics. The session included an in-depth discussion regarding the importance of suitability testing, and a comparison of USP methodology to AOAC/FDA BAM methodology. USP also provided an update on new USP chapters and shared insight on what is on the horizon for USP methods.

Keywords: Webinar Microbiology Hemp & CBD Probiotics Supplements

Identification of Probiotic Organisms by Whole Genome Sequencing

DNA sequencing is an important tool to identify all Probiotic organisms in a sample all in one assay. In this Scientific Poster, Eurofins scientists investigate a long-read sequencing method that accurately identifies probiotic organisms in complex samples recently presented at SHIFT 20.

Keywords: Scientific Research Microbiology Probiotics