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Ethylene oxide is gaining attention in the food regulatory space. EtO was banned as a food fumigant in 1991 in the EU. While ethylene oxide is not banned for use in foods in the United States, it is important to understand the allowed tolerances in the US and EU, especially as regulations continue to evolve and online sales contribute to increasing overseas exports. This article outlines the uses and toxicity of EtO, US and EU regulations, and EtO analysis at Eurofins.