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Andrzej Benkowski, Technical Manager, Eurofins Microbiology Laboratories Inc, is one of the authors of this article from Frontiers in Microbiology, discussing enumeration techniques for probiotic and postbiotic analytical methods.


In a recent webinar, experts answered some FAQs about probiotic enumeration, with a focus on the applications of flow cytometry to probiotic products. This blog provides an overview of the questions and answers, including the benefits of flow cytometry, how it compares to plate counts, and considerations for testing.


This is a follow up on our very successful previous webinar session where we discussed what is flow cytometry, advantages and disadvantages of flow cytometry, and marketplace examples and investigative case studies. Original airdate August 8, 2023.


Flow cytometry provides rapid, accurate, and powerful results for probiotic brands and manufactures. This case study demonstrates how flow cytometry can assist with low-cell count investigations by reveling the number of live, injured, and dead cells.


Probiotic enumeration is a key component of a probiotic testing scheme. Traditional plate count methods can provide inaccurate results, especially considering non-culturable cells. This case study demonstrates how flow cytometry can determine an accurate cell count, even of non-culturable, live cells.


Combining multiple strains of bacteria in a single probiotic product is a popular way to increase its health benefits. Flow cytometry provides a method for enumerating individual strains or species with accurate total bacteria counts. This case study demonstrates how flow cytometry can assist with multi-species enumeration when plate count methods provide low counts.


Microencapsulated cells provide a way to introduce probiotics into new products, like drinks. However, plating methods often fail to recover live cells in this form. Flow cytometry provides rapid, accurate, and powerful results for the enumeration of microencapsulated probiotics. This case study demonstrates how flow cytometry can assist with microencapsulated enumeration in a drink product.


Postbiotics are increasing in popularity, but are difficult to enumerate with traditional plating methods. Flow cytometry provides an avenue for measuring the dead or inactive cells in postbiotic products. This case study demonstrates how flow cytometry can assist with postbiotic enumeration when plate methods result in low cell counts.


This webinar discusses what is flow cytometry, advantages and disadvantages of flow cytometry, and marketplace examples and investigative case studies. Original airdate June 27, 2023.


In this webinar we review the call to action from the FDA, provide an overview of the technology of whole genome sequencing. Discuss the benefits of whole genome sequencing, and review the ways that Eurofins is ready to support your testing needs. Original Air Date May 18, 2023


 

 

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