Rapid and Economic Determination of 13 Steviol Glycosides in Market-Available Food, Dietary Supplements, and Ingredients
Abstract
Steviol glycosides, obtained from leaves of Stevia rebaudiana Bertoni (stevia) or produced via bioconversion and biosynthesis, are diterpenes used by the food/dietary supplement industry as zero-calorie sweeteners derived from natural sources.
To address the industrial needs for rapid and economic analysis of steviol glycoside products, we developed and validated a gradient HPLC-UV method using a superficially porous particle reversed-phase C18 column to separate 13 steviol glycosides. The validation results showed that the proposed method was specific, suitable, linear, accurate, and precise. This method has the potential to be adapted as an industrial standard for the rapid and economic analysis for testing steviol glycosides.
Eurofins Scientific Director, Dr. Hong You, co-authors in this novel research.
