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Food Testing >> Resources >> Probiotic Yeast Enumeration by Flow Cytometry: Method Modification Verification of ISO 19344(B)

Probiotic Yeast Enumeration by Flow Cytometry: Method Modification Verification of ISO 19344(B)

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Increasingly, probiotic dietary supplement stakeholders are using flow cytometry to address manufacturers’ and consumers’ demands for information about the quantity, quality, and identity of organisms in probiotic products. Previously, we verified ISO 19344:2015(E)/IDF 232:2015(E) for enumerating probiotic bacteria in lyophilized powders using acoustic focusing flow cytometry. The study showed that cytometric accuracy, precision, and turnaround times were comparable to or better than those of plate and microscopic counting. Here, the utility of flow cytometry is expanded further by applying modifications to ISO 19344 (Protocol B) in order to accurately quantify the yeast Saccharomycescerevisiae var. boulardii.

Authors:

Andrzej A. Benkowski, Nathaniel O. Tull and J. David Legan

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