Pathogen Detection by Loop-Mediated Amplification - Brown, et al
Pathogen detection in foods and environmental samples is a critical element in a food management system. Speed, sensitivity, and precision are of vital importance to manufacturers of perishable foods, and PCR has become an industry standard because it has these attributes. Unfortunately, many food components, particularly from spices and botanicals, inhibit the amplification cycle in PCR. This study set out to compare the robustness of Loop Mediated Amplification (LAMP) with that of PCR as illustrated by the detection threshold of Listeria and Salmonella spiked into culture broths previously incubated with various spices.
