Determination of Cannabinoids in Plant Materials: Laboratory Validation for AOAC First Action Official Method Consideration
Overview
In the last few years, the popularity of Cannabis plants and Cannabis-based products has significantly increased as many countries around the globe have adopted a more liberal view towards the use of this plant for medicinal purposes. Among more than 500 compounds identified in Cannabis plants, most attention has been paid to cannabinoids, especially Δ9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) due to their biological activities.
In this study, a single-laboratory validation (SLV) of ultra-high performance liquid chromatography–diode array detection (UHPLC–DAD) method for quantification of twelve major cannabinoids in Cannabis dried plant materials, concentrates and oils, was performed. The obtained results demonstrate applicability of the method to quantitative analysis of important cannabinoids evaluated matrices. The performance of the method passes performance requirements defined by AOAC Standard Method Performance Requirements (SMPRs) 2017.001 and 2017.002.
Authors
Lukas Vaclavik of Eurofins Food Integrity & Innovation (EFII) in North Yorkshire, UK; Frantisek Benes, Marie Fenclova, Jiri Hricko, Ales Krmela, Veronika Svobodova, and Jana Hajslova of EFII in Prague, Czech Republic and Katerina Mastovska of EFII in Madison, WI, USA.
