Determination of Active Vitamin B12 (Cobalamin) in Dietary Supplements and Ingredients by Reverse-phase Liquid Chromatography: Single-laboratory Validation - Qiu, et al
Minority populations, such as vegan/vegetarian, pregnant women, infants, and eating disorder patients, can be vulnerable to vitamin B12 deficiency because of their unique dietary habits and physiological needs. Vitamin B12 dietary supplement was recognized as an alternative food nutrient resource for groups at risk of vitamin B12 deficiency. This study seeks to develop and validate a method for identifying and quantifying four bioactive forms of vitamin B12 (adenosylcobalamin, cyanocobalamin, hydroxocobalamin, and methylcobalamin) from dietary ingredients and supplements, as a consensus quality control protocol for dietary supplement labeling and manufacturing.
