J. David Legan, PhD
Director of Science
David earned his Ph.D. in Food Technology from the University of Reading in the UK by modeling the ecology of mixed microbial populations, and then moved to Campden BRI in a variety of microbiological food safety research and client service roles. During that time, he was project lead for the Bacillus component of the UK’s pathogen modeling program. He moved again to Nabisco Research in New Jersey where he ran the corporate microbiology lab and developed a program of preservation technology development and microbial modeling. After the Kraft Foods acquisition, he moved to Chicago to work on Food Safety and Preservation research, and through modeling and validation studies:
- Optimized Oscar Mayer’s use of lactate and diacetate and their naturally cultured alternatives as Listeria-control agents in Ready to Eat meats
- Specified process conditions central to Oscar Mayer’s commercial launch of High Pressure Pasteurization of naturally cured RTE meats
David had responsibility for the Kraft cultures R&D group, developed a partnership to explore microwave sterilization leading to several patents, and led a program that developed an internal proprietary natural antimicrobial commercialized in several Kraft products. Technologies from his group supported approximately $4 billion in annual sales.
After years as a microbiology "client", he is now back in the "provider" role as Director of Science at Eurofins Microbiology Laboratories, Inc., by way of the Covance Food Solutions group based in Madison, WI, which he joined in 2016. In this role, he ensures appropriate method validation, explores new testing technologies, and fields multiple complicated food microbiology questions.
Products that his team has evaluated or developed and launched include:
- The 3M MDS platform in the Madison microbiology laboratory
- Flow cytometry for enumeration of probiotics
- Strain-level confirmation of probiotic identification using the polymerase chain reaction (PCR)
- Next-generation sequencing using the Oxford Nanopore Technologies GridION sequencing platform for microbial identification and microbiome analysis
Below are resources from David:
Food fraud, also known as economically motivated adulteration, is widespread worldwide. Food fraud involves deliberate and intentional substitution, addition, tampering or misrepresentation of food, food ingredients or food packaging, labeling, product information or false or misleading statements made about a product for economic gain. Food fraud can adversely impact consumer health, product quality, and brand reputation.
Validation of aseptic fillers intended for the production of low-acid, shelf-stable products is a core requirement of the FDA’s low-acid canned food (LACF) regulations (21CFR113 and 108.35). Microbiological challenges of the aseptic zone and packaging material sterilization cycles are important components of the validation process. These challenges are often conducted at the production site using non-pathogenic surrogate organisms that mimic the resistance of the pertinent target pathogen. This presentation will provide a brief history of the use of such surrogates for the validation of low-acid aseptic fillers along with the approach we have used to qualify the surrogates as adequate for the intended technology.”
When we send a sample of food for testing, we generally think about a pretty simple system where we submit a sample, wait a bit of time, and then find out if the target organism is there. What we often forget is just how complex our food, processing systems, and environments truly are.
Today, there is a widely shared belief that the rate of non confirming presumptives (NCP’s) “false positives” in food pathogen diagnostics has increased significantly in recent years. This increase has been most closely associated with PCR and real time PCR testing. It has not yet been possible to answer the question definitively, nevertheless, the belief is real, and the limited data that have been analyzed suggest that non confirming presumptive rates are indeed on the rise.
Food and supplement testing has been a necessary part of the safe supply chain which means so have the chemicals that go along with this and until today, there have been very few options that positively affect the environment. Supercritical Fluid Extraction with Supercritical Fluid Chromatography now offers an environmentally safer method that is also faster, as sensitive and as selective as your current methods. We present an alternative way to extract and measure vitamins.
Join our team of presenters at Eurofins Microbiology Laboratories for an in-depth webinar all about rapid microbiological methods. They presented on the range of methods available, as well as the technologies behind them, while also exploring factors that affect their performance. Learn what to consider when selecting a rapid method such as sensitivity, validation status, and speed. Practical considerations around the method applications will be addressed.
The reclassification of the genus Lactobacillus into 25 different genera has direct implications for the probiotic industry. This webinar describes the rationale behind the name change, what it means for the industry, how the taxonomy update will be implemented, and why it is a good thing for the consumer.
Do your products need a nutritional label? Do you ever find yourself facing confusion around nutritional labeling requirements?
Let us help with those questions! Listen to our informational webinar presented by Mollie Hammerschmidt, Analytical Services Manager, Eurofins Nutrition Analysis Center. She answers the most common questions and pain points we receive, from customers just like you, with nutrition labeling requests.
Are you stuck on the proverbial micro testing loop? Do you submit a sample to the lab, get a non-compliant result, fill out some paperwork, visit a field and then just start right back over at step one? If so watch this webinar on how to fix those issues.
You walk the field, you pick some product, and you drop it off at the lab for testing. Not long after, you hear back your sample is presumptive. Now what?
