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J. David Legan

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J. David Legan, PhD

J. David Legan, PhD

Director of Science

David earned his Ph.D. in Food Technology from the University of Reading in the UK by modeling the ecology of mixed microbial populations, and then moved to Campden BRI in a variety of microbiological food safety research and client service roles. During that time, he was project lead for the Bacillus component of the UK’s pathogen modeling program.  He moved again to Nabisco Research in New Jersey where he ran the corporate microbiology lab and developed a program of preservation technology development and microbial modeling.  After the Kraft Foods acquisition, he moved to Chicago to work on Food Safety and Preservation research, and through modeling and validation studies:

  • Optimized Oscar Mayer’s use of lactate and diacetate and their naturally cultured alternatives as Listeria-control agents in Ready to Eat meats
  • Specified process conditions central to Oscar Mayer’s commercial launch of High Pressure Pasteurization of naturally cured RTE meats

David had responsibility for the Kraft cultures R&D group, developed a partnership to explore microwave sterilization leading to several patents, and led a program that developed an internal proprietary natural antimicrobial commercialized in several Kraft products. Technologies from his group supported approximately $4 billion in annual sales.

After years as a microbiology "client", he is now back in the "provider" role as Director of Science at Eurofins Microbiology Laboratories, Inc., by way of the Covance Food Solutions group based in Madison, WI, which he joined in 2016.  In this role, he ensures appropriate method validation, explores new testing technologies, and fields multiple complicated food microbiology questions.

Products that his team has evaluated or developed and launched include:

  • The 3M MDS platform in the Madison microbiology laboratory
  • Flow cytometry for enumeration of probiotics
  • Strain-level confirmation of probiotic identification using the polymerase chain reaction (PCR)
  • Next-generation sequencing using the Oxford Nanopore Technologies GridION sequencing platform for microbial identification and microbiome analysis

 

Below are resources from David:



In this white paper, you'll learn more about the details of Section 402(a)(1) of the Food Drug and Cosmetic Act that defines adulteration. What is considered contamination? How it can happen. What levels of extraneous material are acceptable? Filth analysis should be part of any HACCP plan that requires examination of raw material.


Eurofins presents a validation of USP <467> residual solvent methodology for testing on CBD and hemp-based products at the North American Chemical Residue Workshop.


Eurofins presents at AOAC Annual Meeting and Exposition in 2020 summarizing the modifications made to the AOAC Official Method 2018.11 for quantification of cannabinoids to include conversion of Δ9-THCA into Δ9-THC.


Eurofins develops and validates a versatile HPLC-UV/DAD method for analyzing twelve major cannabinoids in dried plant materials, concentrates and oils. Method was granted AOAC First Action OMA status (AOAC Official Method 2018.11).


As Minneola, a hybrid between grapefruit and mandarin, has become more popular it may be susceptible to fraud and/or adulteration. This poster shows the phenolic pattern of this fruit, in relation to other orange, grapefruit and mandarin fruits that may be used to identify its juice or juice concentrate.


DNA sequencing is an important tool to identify all Probiotic organisms in a sample all in one assay. In this Scientific Poster, Eurofins scientists investigate a long-read sequencing method that accurately identifies probiotic organisms in complex samples recently presented at SHIFT 20.


Eurofins presents at AOAC 2020 Virtual Annual Meeting & Exposition. Adulteration to fruit juices has been a long-standing problem in food processing. While analytical tests exist to detect cheaper juices in more expensive ones, an ongoing problem arises when lime juice is blended with lemon juice. Due to their chemical similarities, this requires new in-depth methods.


In this poster, Eurofins describes how the utility of flow cytometry was expanded further by applying modifications to ISO 19344(B) in order to accurately quantify the yeast in the sample.


This analysis compares BACGene Real-Time PCR and BAX® System PCR Methods for the Detection of Salmonella in Pet Food.


To address the industrial needs for rapid and economic analysis of steviol glycoside products we developed and validated a method. This method has the potential to be adapted as an industrial standard for the rapid and economic analysis for testing steviol glycosides.


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