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J. David Legan

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J. David Legan, PhD

J. David Legan, PhD

Director of Science

David earned his Ph.D. in Food Technology from the University of Reading in the UK by modeling the ecology of mixed microbial populations, and then moved to Campden BRI in a variety of microbiological food safety research and client service roles. During that time, he was project lead for the Bacillus component of the UK’s pathogen modeling program.  He moved again to Nabisco Research in New Jersey where he ran the corporate microbiology lab and developed a program of preservation technology development and microbial modeling.  After the Kraft Foods acquisition, he moved to Chicago to work on Food Safety and Preservation research, and through modeling and validation studies:

  • Optimized Oscar Mayer’s use of lactate and diacetate and their naturally cultured alternatives as Listeria-control agents in Ready to Eat meats
  • Specified process conditions central to Oscar Mayer’s commercial launch of High Pressure Pasteurization of naturally cured RTE meats

David had responsibility for the Kraft cultures R&D group, developed a partnership to explore microwave sterilization leading to several patents, and led a program that developed an internal proprietary natural antimicrobial commercialized in several Kraft products. Technologies from his group supported approximately $4 billion in annual sales.

After years as a microbiology "client", he is now back in the "provider" role as Director of Science at Eurofins Microbiology Laboratories, Inc., by way of the Covance Food Solutions group based in Madison, WI, which he joined in 2016.  In this role, he ensures appropriate method validation, explores new testing technologies, and fields multiple complicated food microbiology questions.

Products that his team has evaluated or developed and launched include:

  • The 3M MDS platform in the Madison microbiology laboratory
  • Flow cytometry for enumeration of probiotics
  • Strain-level confirmation of probiotic identification using the polymerase chain reaction (PCR)
  • Next-generation sequencing using the Oxford Nanopore Technologies GridION sequencing platform for microbial identification and microbiome analysis

 

Below are resources from David:



Steam volatile oil is an important property of spices. The volatile components present in spices contribute to the aroma and flavor of the spice. This paper describes a fast, accurate, and simple technique for analysis of spices using Fourier Transform Near Infrared (FT-NIR) technology.


Researchers from Eurofins Microbiology and Third Wave Bioactives collaborated to compare the microbial succession of refrigerated chicken noodle soup. The purpose of better understanding how various culture ingredients not only prevent microbial spoilage populations, but also dive deeper into how microbial populations evolve over time.


This white paper explores off-flavor and odor investigations performed by Eurofins SFA. Authored by Eurofins' scientist Jamie Willems Ph.D.


This study seeks to establish the detectability of Listeria species in probiotic cultures and estimate the sensitivity of detection.


This study seeks to develop and validate a method for identifying and quantifying four bioactive forms of vitamin B12 (adenosylcobalamin, cyanocobalamin, hydroxocobalamin, and methylcobalamin) from dietary ingredients and supplements, as a consensus quality control protocol for dietary supplement labeling and manufacturing.


Pathogen detection in foods and environmental samples is a critical element in a food management system. This study set out to compare the robustness of Loop Mediated Amplification (LAMP) with that of PCR as illustrated by the detection threshold of Listeria and Salmonella spiked into culture broths previously incubated with various spices.


Enumeration provides the most basic required information with methodologies evolving to provide improved accuracy, precision, and turnaround times. Acoustic-assisted hydrodynamic flow cytometry incorporates the advance of using sound waves to align the particles in the fluid stream for counting, reducing the time to result.


As probiotic consumption rates continue to grow, stakeholders are demanding more information about the quantity, quality, and identity of organisms in probiotic products. Increasingly, flow cytometry is being used to address manufacturers’ and consumers’ demands, and has shown promise in rapidly providing precise quantitative and qualitative probiotic information.




This poster describes some of the work Eurofins has conducted on procedures to help control the quality of agave syrups.


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