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Non-culturable presumptive (NCP)- samples that yield positive pathogen detection results by PCR screening methods but do not confirm by cultural methods- can be frustrating and disruptive to clients. These situations are not unique to a testing company or the methods that are run, but it is possible to understand the root causes of NCPs, and to identify additional procedures to reduce the frequency with which they occur and minimize the likelihood of these situations impacting your data.